human pulmonary artery fibroblasts hpafs Search Results


human pulmonary artery fibroblasts hpafs  (ScienCell)
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ScienCell human pulmonary artery fibroblasts hpafs
Effects of silencing NHE1 on proliferation of human (HPASMCs), human pulmonary artery endothelial cells (HPAECs), and human pulmonary artery <t>fibroblasts</t> <t>(HPAFs)</t> under condition of hypoxia. (A) Representative RT-PCR data show expression of NHE1 mRNA in three cell types. RNA was isolated from HPASMCs, HPAECs, and HPAFs. RT-PCR was performed to measure the expression of NHE1. GAPDH was used as loading control. (B) Cell proliferation data. After transfection with NHE1 siRNA, HPASMCs, HPAECs, and HPAFs were cultured in a 2% oxygen chamber for 24 hours, and harvested for gene expression and cell counts to assay cell proliferation. *P < 0.05, compared with control group (n = 9 for each group).
Human Pulmonary Artery Fibroblasts Hpafs, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of silencing NHE1 on proliferation of human (HPASMCs), human pulmonary artery endothelial cells (HPAECs), and human pulmonary artery fibroblasts (HPAFs) under condition of hypoxia. (A) Representative RT-PCR data show expression of NHE1 mRNA in three cell types. RNA was isolated from HPASMCs, HPAECs, and HPAFs. RT-PCR was performed to measure the expression of NHE1. GAPDH was used as loading control. (B) Cell proliferation data. After transfection with NHE1 siRNA, HPASMCs, HPAECs, and HPAFs were cultured in a 2% oxygen chamber for 24 hours, and harvested for gene expression and cell counts to assay cell proliferation. *P < 0.05, compared with control group (n = 9 for each group).

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Silencing of Sodium-Hydrogen Exchanger 1 Attenuates the Proliferation, Hypertrophy, and Migration of Pulmonary Artery Smooth Muscle Cells via E2F1

doi: 10.1165/rcmb.2011-0032OC

Figure Lengend Snippet: Effects of silencing NHE1 on proliferation of human (HPASMCs), human pulmonary artery endothelial cells (HPAECs), and human pulmonary artery fibroblasts (HPAFs) under condition of hypoxia. (A) Representative RT-PCR data show expression of NHE1 mRNA in three cell types. RNA was isolated from HPASMCs, HPAECs, and HPAFs. RT-PCR was performed to measure the expression of NHE1. GAPDH was used as loading control. (B) Cell proliferation data. After transfection with NHE1 siRNA, HPASMCs, HPAECs, and HPAFs were cultured in a 2% oxygen chamber for 24 hours, and harvested for gene expression and cell counts to assay cell proliferation. *P < 0.05, compared with control group (n = 9 for each group).

Article Snippet: Cells Human pulmonary artery smooth muscle cells (HPASMCs), human pulmonary artery endothelial cells (HPAECs; Lonza, Inc., Walkersville, MD), and human pulmonary artery fibroblasts (HPAFs; ScienCell Research Laboratories, Carlsbad, CA) were used in this study.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Isolation, Control, Transfection, Cell Culture, Gene Expression